Primer

Part:BBa_K2309032

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)


Standard reverse primer for Gibson Assembly to pNZ8148

This primer contain 17 bp of the standard BioBrick suffix and the rest can be digested and ligated into pNZ8148 at the restriciton site PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None